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Unformatted text preview: 59 35 Mean 6.56 3.89 percentage of polymorphisms per primer ranged from
20% to 77.8% with an average of 55.3%. This indicates
that there was considerable RAPD variation among the
regenerants. Figure 3 shows the amplification profiles
generated by primers OPAW 11, OPU 13, and OPU 16
across the regenerants.
The Jaccard’s coefficient of similarity provided
similarity values ranging from 0.537 to 0.860, indicative
of the level of genetic variation among the potential
mutant lines (Table 3). These coefficients reflected the
genetic relationship between the samples. The highest
level of similarity between 2 potential mutant lines was
found between PL2 and PL3, with a similarity coefficient
of 0.86 (the shortest genetic distance), while a minimum
similarity coefficient (0.628) was observed between PL1
and PL7 (the highest genetic distance). For comparison
between the potential mutant lines and the control, the
maximum similarity value (0.814) was observed in PL1
mutant while the minimum value (0.537) was o...
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- Spring '14