56 389 percentage of polymorphisms per primer ranged

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 59 35 Mean 6.56 3.89 percentage of polymorphisms per primer ranged from 20% to 77.8% with an average of 55.3%. This indicates that there was considerable RAPD variation among the regenerants. Figure 3 shows the amplification profiles generated by primers OPAW 11, OPU 13, and OPU 16 across the regenerants. The Jaccard’s coefficient of similarity provided similarity values ranging from 0.537 to 0.860, indicative of the level of genetic variation among the potential mutant lines (Table 3). These coefficients reflected the genetic relationship between the samples. The highest level of similarity between 2 potential mutant lines was found between PL2 and PL3, with a similarity coefficient of 0.86 (the shortest genetic distance), while a minimum similarity coefficient (0.628) was observed between PL1 and PL7 (the highest genetic distance). For comparison between the potential mutant lines and the control, the maximum similarity value (0.814) was observed in PL1 mutant while the minimum value (0.537) was o...
View Full Document

Ask a homework question - tutors are online