A nonirradiated explant m1v3 was used as the control

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Unformatted text preview: d individually after the third generation (M1V3) in culture. A nonirradiated explant (M1V3) was used as the control. Total DNA was extracted from in vitro young leaves using the method described by Kiefer et al. (2000), with one modification whereby spermidine was omitted. Each YUNUS et al. / Turk J Biol genomic DNA sample was checked for the level of purity and concentration using Nanodrops (Spectrophotometer ND-1000 V3.5, NanoDrop Technologies, Wilmington, DE, USA). DNA was diluted to make a working solution for RAPD marker analysis. The quality of dilution was further checked on 1.0% agarose gel. 2.6. Primer selection A total of 14 primers were used for preliminary amplification and primer selection. Primers that produced reproducible polymorphic bands were selected. The RAPD primers (OPAW 11, OPAW 17, OPU 10, OPU 13, OPU 16, and OPG 14) were randomly chosen based on the study of Phalaenopsis orchids by Goh et al. (2005). Meanwhile, 8 SSR rice primers were tested for a cross amplification study based on...
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This document was uploaded on 02/23/2014.

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