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individually after the third generation (M1V3) in culture.
A nonirradiated explant (M1V3) was used as the control.
Total DNA was extracted from in vitro young leaves
using the method described by Kiefer et al. (2000), with
one modification whereby spermidine was omitted. Each YUNUS et al. / Turk J Biol genomic DNA sample was checked for the level of purity
and concentration using Nanodrops (Spectrophotometer
ND-1000 V3.5, NanoDrop Technologies, Wilmington,
DE, USA). DNA was diluted to make a working solution
for RAPD marker analysis. The quality of dilution was
further checked on 1.0% agarose gel.
2.6. Primer selection
A total of 14 primers were used for preliminary
amplification and primer selection. Primers that produced
reproducible polymorphic bands were selected. The RAPD
primers (OPAW 11, OPAW 17, OPU 10, OPU 13, OPU 16,
and OPG 14) were randomly chosen based on the study
of Phalaenopsis orchids by Goh et al. (2005). Meanwhile,
8 SSR rice primers were tested for a cross amplification
study based on...
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This document was uploaded on 02/23/2014.
- Spring '14