Unformatted text preview: shoots from untreated explants needed only 4
weeks to reach 1 cm. Gamma-ray irradiation did not affect
the ability of the M1V0 shoots to produce adventitious
Attempts to regenerate and multiply irradiated buds
(M1V0) under light conditions were unsuccessful (data
not shown). All irradiated buds became necrotic and
died after a period of 6–8 weeks of incubation under light
conditions. The present study indicated that irradiated
buds only survived and regenerated shoots if the buds
were maintained under dark conditions on a regeneration
medium during the 8 weeks.
3.2. RAPD marker analysis of gamma-ray–treated
Markers were selected based on their ability to generate a
visible polymorphism between the samples. For the RAPD
analysis performed in this study, 9 of the 14 primers tested
gave scorable bands. The 9 primers generated 59 scorable
bands ranging from 300 to 3500 bp in size. Of these, 35
bands (55.31%) were polymorphic (Table 2). The number
of bands for each primer varied...
View Full Document
This document was uploaded on 02/23/2014.
- Spring '14