In this report a technique has been standardized for

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Unformatted text preview: report, a technique has been standardized for the management of chimeric tissues through multiple bud regeneration. Molecular techniques have greatly aided the understanding of plant cell responses to mutation induction. They also provide a better understanding of the potential and limitations of mutation breeding, which can lead to early identification of useful variants. The major advantages of RAPD are the low cost and effort required for its application (Atienzar and Jha, 2002; Dhakshanamoorthy et al., 2011). To our knowledge, this is the first report on in vitro mutagenesis in E. elatior and the application of RAPD markers to detect variations. 2. Materials and methods 2.1. Plant materials Individual buds (±0.5 cm) obtained from 6-month-old axenic cultures (at the multiplication stage) (Yunus et al., 2012) were excised and cultured on MS medium (Murashige and Skoog, 1962) containing 3% sucrose (w/v), 0.4% Gelrite (Duchefa, Haarlem, the Netherlands), 0.1 mg L–1 myo-inositol, and devoid of plant growth regulator. The...
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This document was uploaded on 02/23/2014.

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