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Unformatted text preview: report, a technique has been standardized for the
management of chimeric tissues through multiple bud
regeneration. Molecular techniques have greatly aided
the understanding of plant cell responses to mutation
induction. They also provide a better understanding
of the potential and limitations of mutation breeding,
which can lead to early identification of useful variants.
The major advantages of RAPD are the low cost and
effort required for its application (Atienzar and Jha, 2002;
Dhakshanamoorthy et al., 2011). To our knowledge, this is
the first report on in vitro mutagenesis in E. elatior and the
application of RAPD markers to detect variations.
2. Materials and methods
2.1. Plant materials
Individual buds (±0.5 cm) obtained from 6-month-old
axenic cultures (at the multiplication stage) (Yunus et
al., 2012) were excised and cultured on MS medium
(Murashige and Skoog, 1962) containing 3% sucrose (w/v),
0.4% Gelrite (Duchefa, Haarlem, the Netherlands), 0.1 mg
L–1 myo-inositol, and devoid of plant growth regulator.
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This document was uploaded on 02/23/2014.
- Spring '14