Morphological abnormality appeared on some of the

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Unformatted text preview: the lower dose of 10 Gy (Figure 2A) and the control treatment. Morphological abnormality appeared on some of the irradiated explants (Figure 2B). Meanwhile, no abnormality in morphology appeared for the surviving irradiated explants. In addition, all explants irradiated beyond 20 Gy failed to survive after the third subculture (12 weeks of culture) (Figure 2C), and all morphologically defective explants failed to grow. No callus was formed during the 8-week incubation period. A B C D Figure 2. Effect of different levels of gamma irradiation on in vitro buds of E. elatior. A) Survival of explants exposed to 10 Gy after 8 weeks of culture. B) Morphological abnormality observed in irradiated explant which later died after M1V1 stage. C) Explant irradiated with 20 Gy failed to survive after the third subculture. D) M1V1 shoots produced from 10 Gy irradiated explant after 12 weeks of culture. Bar = 1 cm. In this study, the gamma-ray–treated buds were cultured under optimal conditions for shoot regener...
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This document was uploaded on 02/23/2014.

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