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Unformatted text preview: the lower dose of 10 Gy (Figure 2A) and the
control treatment. Morphological abnormality appeared
on some of the irradiated explants (Figure 2B). Meanwhile,
no abnormality in morphology appeared for the surviving
irradiated explants. In addition, all explants irradiated
beyond 20 Gy failed to survive after the third subculture
(12 weeks of culture) (Figure 2C), and all morphologically
defective explants failed to grow. No callus was formed
during the 8-week incubation period. A B C D Figure 2. Effect of different levels of gamma irradiation on in vitro
buds of E. elatior. A) Survival of explants exposed to 10 Gy after
8 weeks of culture. B) Morphological abnormality observed in
irradiated explant which later died after M1V1 stage. C) Explant
irradiated with 20 Gy failed to survive after the third subculture.
D) M1V1 shoots produced from 10 Gy irradiated explant after 12
weeks of culture. Bar = 1 cm. In this study, the gamma-ray–treated buds were
cultured under optimal conditions for shoot regener...
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This document was uploaded on 02/23/2014.
- Spring '14