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Unformatted text preview: ocedures could be done in just 6–7 months by
using in vitro techniques, whereas it is estimated that the
conventional field process needs more than a year to build up
a population for screening gamma-ray–irradiated suckers.
Thus, in vitro mutagenesis saves time, equipment, cost, and
space. Additionally, in vitro selection allows researchers to
control the experimental environment more precisely.
The decline and lower rate of regeneration of irradiated
explants at different doses as observed in the present study
could be attributed to intrasomatic competition (diplontic
selection) in the irradiated multicellular layers. In chimeric
tissue, mutated cells are present along with the normal
cells. During subsequent cell division, the mutated cells
compete with the surrounding normal cells for survival 7 YUNUS et al. / Turk J Biol (Datta et al., 2005). Thus, the low rate of irradiated explant
recovery may be due to replacement of mutated sectors
with normal cells, reduced competitiveness of mutated
cells, and prevention of the appearance of mutated cells
(Mandal et al., 200...
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- Spring '14