Such procedures could be done in just 67 months by

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Unformatted text preview: ocedures could be done in just 6–7 months by using in vitro techniques, whereas it is estimated that the conventional field process needs more than a year to build up a population for screening gamma-ray–irradiated suckers. Thus, in vitro mutagenesis saves time, equipment, cost, and space. Additionally, in vitro selection allows researchers to control the experimental environment more precisely. The decline and lower rate of regeneration of irradiated explants at different doses as observed in the present study could be attributed to intrasomatic competition (diplontic selection) in the irradiated multicellular layers. In chimeric tissue, mutated cells are present along with the normal cells. During subsequent cell division, the mutated cells compete with the surrounding normal cells for survival 7 YUNUS et al. / Turk J Biol (Datta et al., 2005). Thus, the low rate of irradiated explant recovery may be due to replacement of mutated sectors with normal cells, reduced competitiveness of mutated cells, and prevention of the appearance of mutated cells (Mandal et al., 200...
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