The ld50 is defined as the dose corresponding to a 50

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Unformatted text preview: egeneration rates of buds after 8 weeks in culture are summarized in Figure 1. The LD50 is defined as the dose corresponding to a 50% decrease in the control regeneration percentage. In this experiment, the 50% lethal dose of explants 8 weeks after irradiation (LD50) was estimated to be 10 Gy, and doses beyond 80 Gy did not show any signs of regeneration (Figure 1). The regeneration of irradiated shoots was strongly influenced by the radiation dose. Higher doses inhibit regeneration, whereby gamma irradiation in excess of a critical dose caused obvious depression 100 Surv val rate of mult ple buds (%) 90 80 70 60 50 40 30 20 10 0 0 10 20 30 40 50 60 70 80 90 Actual dose (Gy) 100 110 120 130 140 150 Figure 1. LD50 determination in survival rate of irradiated buds of E. elatior after 8 weeks of incubation. 3 YUNUS et al. / Turk J Biol in multiple bud growth. Treatments as low as 10 Gy showed irradiation damage; half of the explants turned black, retarded, and finally died. Surviving explants were observed at...
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