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Unformatted text preview: The RAPD-marker–generated genetic variation
among the control and gamma- ray–treated lines will help
distinguish the plants for early selection. The RAPD method
has successfully been used to study genetic variability
between in vitro Rhododendron varieties and mutants,
which were exposed to gamma irradiation doses of 5 and
10 Gy (Atak et al., 2011). Barakat et al. (2010) suggested
that by using RAPD markers, the newly gamma-irradiated
chrysanthemum lines can be easily differentiated from
the in vitro Chrysanthemum morifolium cultivar ‘Delistar
White.’ The RAPD technique has successfully differentiated
Gypsophila paniculata variants obtained through in vitro
mutagenesis from their parent (Barakat and El-Sammak,
2011). Detection of in-vitro- mutagenesis–induced DNA
polymorphisms with RAPD has also been reported in
Saccharum officinarum (Ahmed Khan et al., 2009; Bibi et
al., 2010). These results indicated that the RAPD technique
can be a useful tool to detect variation induced through
mutation under in vit...
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This document was uploaded on 02/23/2014.
- Spring '14