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Unformatted text preview: medium. The irradiated explants were placed in vials
containing 10 mL of MS medium supplemented with 13.32
µM BAP, 3% sucrose, 0.4% Gelrite, and 0.1 mg L–1 myoinositol. The cultures were incubated at 25 ± 1 °C under
dark conditions. Cultures were maintained on the same
medium by regular subculturing at 4-week intervals over
a period of 8 weeks. A radiosensitivity test of the plant was
determined by measuring the survivability of the explant
8 weeks after irradiation. Other parameters observed were
the mean number of shoots and leaves developed from the
irradiated explants and morphological changes observed
in the plantlets. The morphological changes were recorded
visually after 1–8 weeks of culture.
After 8 weeks of culture under dark conditions, each
irradiated bud was transferred to fresh medium with
the same growth regulator composition. The medium
was distributed into jam jars (50 mL each), and a single
irradiated bud was placed in each jar. The cultures were
incubated under reduced light conditions provided by
white fluorescent tubes (21 µmol m–2 s–1) at 26 ± 1 °C
with a 16/8-h photo...
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This document was uploaded on 02/23/2014.
- Spring '14