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regeneration. The irradiation dose LD50, which is not
highly inhibitory to plant development, is recommended
(Zhou et al., 2006).
In this study the gamma ray dose clearly affected shoot
development from in vitro buds of E. elatior. With an
increase in gamma ray dose, the percentage of explants that
produced shoots and leaves decreased. The results showed
that shoot induction and shoot growth of irradiated
explants were reduced sharply upon exposure to 10 Gy,
as compared to the nonirradiated explants, indicating
that in vitro buds of E. elatior are very sensitive to gamma
radiation. Lu et al. (2007) found that the LD50 of in vitro
Narcissus tazetta var. chinensis (Chinese narcissus) bulbscales irradiated by gamma rays was similar to E. elatior (10 5 YUNUS et al. / Turk J Biol
M C PL1 A PL1 PL2 PL3 PL4 PL5 PL6 M PL2 PL3 PL4 PL5 PL6 PL7 PL8 PL6 PL7 PL8 PL9 10000 bp 3000 bp 3000 bp 3000 bp 1000 bp
1000 bp PL1 PL2 PL3 PL4 PL5 B 10000 bp 10000 bp PL7 PL8 PL9 B 1000 bp 500 bp 500 bp 500 bp M PL1 PL2 PL3 PL4 PL5 C B C PL1 PL2 PL3 PL4 NC PL6 PL7 PL8 PL9 C PL1 PL2 PL3 PL4 NC PL5 PL6 PL7 PL8 M PL5 PL6 PL7 PL8 M C 10000 bp 10000 bp 10000 bp 3000 bp 3000 bp 3000 bp
1000 bp 1000 bp 1000 bp 500 bp 500 bp 500 bp Figure 3. AC PL1 PL2 PL3 PL4 NC PL5 PL6 PL...
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This document was uploaded on 02/23/2014.
- Spring '14