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Unformatted text preview: pH of the medium was adjusted to 5.7–5.8 before
autoclaving at 121 °C for 15 min. Five buds were cultured
in each petri dish. Cultures were incubated for 3 days at 25
± 1 °C under dark conditions prior to irradiation.
2.2. Gamma irradiation of individual buds
The irradiation process was carried out at the gamma ray
laboratory, School of Applied Physics, Faculty of Science,
Universiti Kebangsaan Malaysia (UKM), Selangor,
Malaysia. The individual buds in petri dishes were
irradiated with 60Co source (Gammacell 220 Excel Co-60,
Nordion, Canada) (dose rate: 2.8741 Kgy/-h) at different
doses: 0 (control), 10, 20, 40, 60, 80, 100, 120, and 140
Gy. One Gy equals the absorption of 1 joule of energy per
kilogram of product irradiated. There were 20 replications
per dose, and each replication consisted of 1 explant. After
irradiation, the irradiated explants were maintained in the
same media in the petri dish and incubated at 25 ± 1 °C
under dark conditions. 2 2.3. Postmutagenesis handling
After 3 days of incubation, the irradiated explants
(designated M1V0) were transferred to shoot multiplication...
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This document was uploaded on 02/23/2014.
- Spring '14