The ph of the medium was adjusted to 5758 before

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Unformatted text preview: pH of the medium was adjusted to 5.7–5.8 before autoclaving at 121 °C for 15 min. Five buds were cultured in each petri dish. Cultures were incubated for 3 days at 25 ± 1 °C under dark conditions prior to irradiation. 2.2. Gamma irradiation of individual buds The irradiation process was carried out at the gamma ray laboratory, School of Applied Physics, Faculty of Science, Universiti Kebangsaan Malaysia (UKM), Selangor, Malaysia. The individual buds in petri dishes were irradiated with 60Co source (Gammacell 220 Excel Co-60, Nordion, Canada) (dose rate: 2.8741 Kgy/-h) at different doses: 0 (control), 10, 20, 40, 60, 80, 100, 120, and 140 Gy. One Gy equals the absorption of 1 joule of energy per kilogram of product irradiated. There were 20 replications per dose, and each replication consisted of 1 explant. After irradiation, the irradiated explants were maintained in the same media in the petri dish and incubated at 25 ± 1 °C under dark conditions. 2 2.3. Postmutagenesis handling After 3 days of incubation, the irradiated explants (designated M1V0) were transferred to shoot multiplication...
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This document was uploaded on 02/23/2014.

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