Then the cultures were incubated under full light

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Unformatted text preview: period (light/dark) for a week. Then the cultures were incubated under full light conditions for 3 subsequent subcultures at 26 ± 1 °C with a 16/8-h photoperiod (light/dark) (30 µmol m–2 s–1) provided by white fluorescent tubes. When the new shoots that emerged (designated M1V1) were 1 cm in length, they were excised from the irradiated shoot clumps (M1V0) and subcultured singly onto fresh medium of the same hormone composition (MS + 13.32 µM of BAP) at 4-week intervals for shoot multiplication through the second and third regeneration cycle (M1V2 and M1V3). Cultures were maintained under full light conditions at 26 ± 1 °C with a 16/8-h photoperiod (light/ dark) (30 µmol m–2 s–1) provided by white fluorescent tubes. 2.4. Experimental design and statistical analysis Randomized complete block design (RCBD) was used in all experiments. Data were subjected to a Kruskal–Wallis test (nonparametric test) for the normality test of the treatment means. Data were subjected to analysis by using SPSS version 18. 2.5. Polymerase chain reaction (PCR) and primer screening A total of 8 regenerants, chosen at random from the healthy growing irradiated explants, were analyze...
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This document was uploaded on 02/23/2014.

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