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Unformatted text preview: period (light/dark) for a week. Then
the cultures were incubated under full light conditions
for 3 subsequent subcultures at 26 ± 1 °C with a 16/8-h
photoperiod (light/dark) (30 µmol m–2 s–1) provided by
white fluorescent tubes.
When the new shoots that emerged (designated M1V1)
were 1 cm in length, they were excised from the irradiated
shoot clumps (M1V0) and subcultured singly onto fresh
medium of the same hormone composition (MS + 13.32
µM of BAP) at 4-week intervals for shoot multiplication
through the second and third regeneration cycle (M1V2
and M1V3). Cultures were maintained under full light
conditions at 26 ± 1 °C with a 16/8-h photoperiod (light/
dark) (30 µmol m–2 s–1) provided by white fluorescent
2.4. Experimental design and statistical analysis
Randomized complete block design (RCBD) was used in
all experiments. Data were subjected to a Kruskal–Wallis
test (nonparametric test) for the normality test of the
treatment means. Data were subjected to analysis by using
SPSS version 18.
2.5. Polymerase chain reaction (PCR) and primer
A total of 8 regenerants, chosen at random from the
healthy growing irradiated explants, were analyze...
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This document was uploaded on 02/23/2014.
- Spring '14