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Unformatted text preview: efore,
consideration must be given to plant material treatment
conditions such as light conditions, regeneration media,
and the PGR used in this experiment. Several modifications
from previous proliferation conditions (Yunus et al.,
2012) that could eliminate the stress were attempted.
The full light conditions clearly affected multiple shoot
development from the irradiated in vitro buds. Complete
absence of light was necessary for shoot induction after
irradiation, indicating that irradiated shoots are sensitive
to light. The appearance of variation might be related to
the stress suffered by the explants postirradiation (Martin
et al., 2002). The fact that some mutant shoots died could
be due to stress, irreversible injuries, local disruption,
and unsuitable conditions for regeneration provoked by
gamma irradiation that may exceed the cell’s capacity to
repair the damage. Intrasomatic competition could be
controlled by modifying the light conditions which may
result in enhanced competitiveness in irradiated cells.
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This document was uploaded on 02/23/2014.
- Spring '14