Lecture 3--cell signaling

do two proteins physically interact in the

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Unformatted text preview: sence of a signal? --Does a signal change the localization of components in the signal transduction pathway? --Does a signal lead to covalent modification of a protein? --Does the signal alter transcription and, if so, what transcripts? Some examples of techniques for studying signal transduction --What proteins participate in a signal transduction pathway? Genetic mutants and RNAi --Do two proteins physically interact in the presence/absence of signal? --Does a signal change the localization of components in the signal transduction pathway. --Does a signal lead to covalent modification of a protein? --Does the signal alter transcription and, if so, what transcripts? Mutant screens in model organisms have led to the discovery of many signal transduction components WT sevenless mutant sevenless over-expression Components of receptor tyrosine kinase signaling pathways were discovered by studying development of the fly eye Mutants in worms and flies defined the components of RTK pathways Components of yeast MAP kinase pathways were discovered by their inability to mate or survive in high osmolarity media Park SH, Zarrinpar A, Lim WA. (2003). Rewiring MAP kinase pathways using alternative scaffold a...
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