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--Does the signal alter transcription and, if so, what
transcripts? Signaling pathways often involve changes in the
phosphorylation states of proteins Antibodies may recognize specific
phosphorylation states of proteins
Phospho-specific antibody Standard antibody antibody to unphosphorylated
protein Antibodies can recognize the phosphorylation
state of a protein Tyrosine hydroxylase antibodies A VEGF receptor antibody Decursin inhibits retinal neovascularization via suppression of VEGFR-2 activation.
Kim JH, Kim JH, Lee YM, Ahn EM, Kim KW, Yu YS. Mol Vis. 2009 Sep 12;15:1868-75. presence or absence of ligand Does internalization activate or shut off VEGFR
Ab staining VEGFR is phosphorylated when activated Lampugnani et al, 2006, JCB 174: 593-604. Some examples of techniques for
studying signal transduction
--What proteins participate in a signal transduction pathway?
--Do two proteins physically interact in the presence/absence
--Does a signal change th...
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- Winter '08
- Cell Signaling