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mechanisms. Science 299(5609):1061-4. Nadal et al. (2004).The MAPK Hog1
recruits Rpd3 histone deacetylase
to activate osmoresponsive genes
Nature 427, 370-374. RNA interference: A technique for knocking down gene function RNA interference exploits native cellular machinery to
causes degradation or silencing of specific transcripts Some examples of techniques for
studying signal transduction
--What proteins participate in a signal transduction pathway?
--Do two proteins physically interact in the presence/absence
--Does a signal change the localization of components in the
signal transduction pathway.
--Does a signal lead to covalent modification of a protein?
--Does the signal alter transcription and, if so, what
transcripts? Testing for interactions by immunoprecipitation Co-localization and Co-IP show that proteins
interact The PAF1 complex differentially regulates cardiomyocyte specification.
Langenbacher AD, Nguyen CT, Cavanaugh AM, Huang J, Lu F, Chen JN. Dev Bi...
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- Winter '08
- Cell Signaling