Unformatted text preview: ol. 2011 (1):19-28. Some examples of techniques for
studying signal transduction
--What proteins participate in a signal transduction pathway?
--Do two proteins physically interact in the presence/absence
--Does a signal change the localization of components in the
signal transduction pathway.
immunofluorescence or fluorescent fusions
--Does a signal lead to covalent modification of a protein?
phos[hospecific antibodies --Does the signal alter transcription and, if so, what
transcripts? Relocalization of a receptor upon signaling: VEGF is a signal
that activates its receptor VEGFR to promote blood vessel
growth presence or absence of ligand cells at varying density Experiment:
1. Vascular cells exposed to
the signal VEGF
2. Cells are stained with an
3. Antibody is chemically
stripped off the surface so that
only internalized VEGFR is
Nucleus = blue (DAPI)
VEGFR = green (Antibody stain)
Lampugnani et al, 2006, JCB 174: 593-604. Relocalization of a receptor upon signaling: VEGF is a signal
that activates i...
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- Winter '08
- Cell Signaling