Lecture 3--cell signaling

Langenbacher ad nguyen ct cavanaugh am huang j lu f

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Unformatted text preview: ol. 2011 (1):19-28. Some examples of techniques for studying signal transduction --What proteins participate in a signal transduction pathway? --Do two proteins physically interact in the presence/absence Co-IP of signal? --Does a signal change the localization of components in the signal transduction pathway. immunofluorescence or fluorescent fusions --Does a signal lead to covalent modification of a protein? phos[hospecific antibodies --Does the signal alter transcription and, if so, what transcripts? Relocalization of a receptor upon signaling: VEGF is a signal that activates its receptor VEGFR to promote blood vessel growth presence or absence of ligand cells at varying density Experiment: 1. Vascular cells exposed to the signal VEGF 2. Cells are stained with an anti-VEGFR antibody 3. Antibody is chemically stripped off the surface so that only internalized VEGFR is visible Nucleus = blue (DAPI) VEGFR = green (Antibody stain) Lampugnani et al, 2006, JCB 174: 593-604. Relocalization of a receptor upon signaling: VEGF is a signal that activates i...
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.

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