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Lecture 3--cell signaling

Relocalization of a receptor upon signaling vegf is a

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Unformatted text preview: ts receptor VEGFR to promote blood vessel growth presence or absence of ligand cells at varying density Experiment: 1. Vascular cells exposed to the signal VEGF 2. Cells are stained with an anti-VEGFR antibody 3. Antibody is chemically stripped off the surface so that only internalized VEGFR is visible What conditions cause VEGFR to be internalized? Lampugnani et al, 2006, JCB 174: 593-604. Nuclear translocation of a MAPK protein followed with immunofluorescence Two co-existing mechanisms for nuclear import of MAP kinase: passive diffusion of a monomer and active transport of a dimer Makoto Adachi, Makoto Fukuda and Eisuke Nishida (1999) EMBO Journal Some examples of techniques for studying signal transduction --What proteins participate in a signal transduction pathway? --Do two proteins physically interact in the presence/absence of signal? --Does a signal change the localization of components in the signal transduction pathway. --Does a signal lead to covalent modification of a protein? phospho-specific antibodi...
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