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Unformatted text preview: ts receptor VEGFR to promote blood vessel
growth presence or absence of ligand cells at varying density Experiment:
1. Vascular cells exposed to
the signal VEGF
2. Cells are stained with an
3. Antibody is chemically
stripped off the surface so that
only internalized VEGFR is
What conditions cause VEGFR to be internalized?
Lampugnani et al, 2006, JCB 174: 593-604. Nuclear translocation of a MAPK protein followed with
immunofluorescence Two co-existing mechanisms for nuclear import of MAP kinase: passive diffusion of a
monomer and active transport of a dimer
Makoto Adachi, Makoto Fukuda and Eisuke Nishida (1999) EMBO Journal Some examples of techniques for
studying signal transduction
--What proteins participate in a signal transduction pathway?
--Do two proteins physically interact in the presence/absence
--Does a signal change the localization of components in the
signal transduction pathway.
--Does a signal lead to covalent modification of a protein?
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- Winter '08
- Cell Signaling