Lecture 3--cell signaling

Even though both exposed to alpha factor not good

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Unformatted text preview: ging that way the cell functions. y-axis shows relative expression of all genes in wt with high salt or pheromone; x-axis shows gene expression in diverter activated with pheromone These days transcription can be assayed by brute force sequencing of all the mRNAs expressed in a cell: RNA-Seq Cells mRNA cDNA library easier and more quantitative take cells and make mRNA from them, and turn into cDNA library, but then cut that up into little small bits, put linkers on them, just prut forht seuences of alll mRNAs transcribed. hard part is that you have to take the bits of pieces and match them up with the mRNA millions of sequences Sequencing Signal transduction 1. General principles of signal transduction 2. Some techniques for studying signal transduction 3. Intracellular receptors: Steroid hormone pathways 4. Second messengers in signal transduction: G-protein 3-5: illustrate many paragmatic procceses you see in signaling transduction pathways, olike existance of second messangers. they are all important in human health 5. Enzyme-linked signal transduction: Tyrosine kinase receptor signaling 6. Pho...
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.

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