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Unformatted text preview: cleotides for once sample and
green for another sample. now you have library of cDNA for both
cells. now you use a chip that contains all the complementary cDNA
sequences hybridize cdna libraries at same time, to microchips that
have probes for all genes. cDNA bind to cmpelementary
probes, and take chip ad makes an abstract art,series of
dots. if see yellow, both were expressed, red spot gene
expressed more in cancer, green expressed in normal.
black spot neither is expressing gene well. How close is the diverter-mediated response to a normal
What are all the genes activated in these scenarios?
have two different kinds of cell.
Perform a micro-array diagonal line tells us
that change in
expression in wild
type is similar to
diverter with alpha
has similar effects
on gene expression
globally as wild type
with osmo. when
you have diverter,
tricked cell into
thinking alpha facotr
is like an osmo
shock eahch dot represents one gene
and how much it changes in each
even though both
exposed to alpha factor
not good correlaition
between the two
situations. th diverter is
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.
- Winter '08
- Cell Signaling