Lecture 6--the cytoskeleton-1

Mitosis and meiosis microtubules form the spindle

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Unformatted text preview: sis Microtubules form the spindle: Dividing sand dollar embryo--microtubules and DNA labeled 6. Mitosis and meiosis Actin plays a role in cytokinesis: Sea Urchin embryos stained for microtubules (white) and myosin II, a component of microfilaments The cytoskeleton 1. Functions of the cytoskeleton: structure, attachment, compartmentalization, motility, transport, cell division 2. Methods for studying the cytoskeleton: EM, antibodies, fusion proteins, drugs, in vitro assays 3. A tour of cytoskeletal filaments: microtubules, intermediate filaments, actin filaments Electron microscopy provides a static, but high resolution view of the cytoskeleton TEM of hemidesmosome with emanating intermediate filaments Electron microscopy provides a static, but high resolution view of the cytoskeleton Platinum Replica Electron Microscopy Tatjana Svitkina Technique: detergent extraction, triple fixation, critical point drying, and shadowing with platinum and carbon Electron microscopy provides a static, but high resolution view of the cytoskeleton Wildtype growth cone Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells. Korobova F, Svitkina T. Mol Biol Cell. 2008 Apr;19(4):1561-74 Electron microscopy provides a static, but high resolution view of the cytoskeleton Arp2/3 deficient growth cone Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells. Korobova F, Svitkina T. Mol Biol Cell. 2008 Apr;19(4):1561-74 Antibodies against components of the cytoskeleton reveal their organization in a cell Figure 16-1 Molecular Biology of the Cell (© Garland Science 2008) Fluorescent fusions to cytoskeletal components has allowed the monitoring of cytoskeletal dynamics in live cells GFP-tubulin fusion GFP-actin fusion Memo-RhoA-mDia1 signaling controls microtubules, the actin network, and adhesion site formation in migrating cells. Zaoui K, Honoré S, Isnardon D,...
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.

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