Unformatted text preview: almon ED. Curr Biol. 1998 Nov
5;8(22):1227-30 Another method for imaging molecular movements: chang
light can actually
eproteing to change
fluorescent properties kaede normaly green,
and flash a light causes
that is irreversible and
turns it red
zebra Hatta K, Tsujii H, Omura T (2006). Cell tracking using a photoconvertible fluorescent protein. Nat
Protoc 1(2):960-7. Another method for imaging molecular movements:
photoconversion Photoconversion of Eos-tubulin makes it possible to image
hat is the rild of the tubulin binding
protein eodtubulin Barlan K, Lu W, Gelfand VI (2013). The microtubule-binding protein ensconsin is an essential
cofactor of kinesin-1. Curr Biol.23(4):317-22. Another method for imaging molecular movements:
just looking at red channel
what is the rold of
microtubule in cell
have moved kinesin and
movement division? knock out the
protein that is
microtubules wont be
made, and you can see
its role in cell division.
what is wrong with this?
tubulin, actin, are so
important in many
functions of the cells that
most cells will start to die
immediately and you
would not see anything.
find ways to affect
functino of components
accutely. one of best
ways to do that is drugs.
becaus e drugs you can
add ata anytime point.
so just put it i before it siRNA against kinesin heavy chain (KHC) and Ensconsin: waht
is the role of these proteins in microtubule dynamics?
Barlan K, Lu W, Gelfand VI (2013). The microtubule-binding protein ensconsin is an essential
cofactor of kinesin-1. Curr Biol.23(4):317-22. Drugs affecting cytoskeletal components in specific ways
facilitate the study of cytoskeletal function reduce dynamics cant grow anymore Table 16-2 Molecular Biology of the Cell (© Garland Science 2008) Drugs affecting cytoskeletal components in specific ways have
advanced the study of the cytoskeleton
stained for tubulin, form
long fibers next to
colchicine colchicine APM no drug destroy cytoskeleton in
APM and coclchine
drugs why would inhibiting microtubule dynamics be good cure for cancer?
want to effect in caner is cell division and it requirs microt...
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- Winter '08
- Proteins, Molecular Biology of the Cell, Microtubule