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Lecture 6--the cytoskeleton-1

Waterman storer cm desai a bulinski jc salmon ed curr

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Unformatted text preview: almon ED. Curr Biol. 1998 Nov 5;8(22):1227-30 Another method for imaging molecular movements: chang light can actually eproteing to change photoconversion fluorescent properties kaede normaly green, and flash a light causes conformational change that is irreversible and turns it red zebra Hatta K, Tsujii H, Omura T (2006). Cell tracking using a photoconvertible fluorescent protein. Nat Protoc 1(2):960-7. Another method for imaging molecular movements: photoconversion Photoconversion of Eos-tubulin makes it possible to image microtubule dynamics hat is the rild of the tubulin binding protein eodtubulin Barlan K, Lu W, Gelfand VI (2013). The microtubule-binding protein ensconsin is an essential cofactor of kinesin-1. Curr Biol.23(4):317-22. Another method for imaging molecular movements: just looking at red channel what is the rold of photoconversion microtubule in cell microtubules have moved kinesin and enscinsin promote movement division? knock out the protein that is component of microtubules, tubulin, microtubules wont be made, and you can see its role in cell division. what is wrong with this? tubulin, actin, are so important in many functions of the cells that most cells will start to die immediately and you would not see anything. find ways to affect functino of components accutely. one of best ways to do that is drugs. becaus e drugs you can add ata anytime point. so just put it i before it siRNA against kinesin heavy chain (KHC) and Ensconsin: waht is the role of these proteins in microtubule dynamics? Barlan K, Lu W, Gelfand VI (2013). The microtubule-binding protein ensconsin is an essential cofactor of kinesin-1. Curr Biol.23(4):317-22. Drugs affecting cytoskeletal components in specific ways facilitate the study of cytoskeletal function reduce dynamics cant grow anymore Table 16-2 Molecular Biology of the Cell (© Garland Science 2008) Drugs affecting cytoskeletal components in specific ways have advanced the study of the cytoskeleton stained for tubulin, form long fibers next to nucleus low colchicine colchicine APM no drug destroy cytoskeleton in APM and coclchine drugs why would inhibiting microtubule dynamics be good cure for cancer? want to effect in caner is cell division and it requirs microt...
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