Lecture 8--intracellular transport

Movement of vesicles in the assay requires atp and

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Unformatted text preview: res ATP and the soluble fraction of axoplasm -Synaptic vesicles added to microtubules in the presence of ATP No binding and no movement -Addition of squid nerve axoplasm preparation Binding and movement observed -Squid axoplasm + microtubules + AMP-PNP Binding but no movement + soluble fraction Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Vale RD, Reese TS, Sheetz MP. Cell. 1985 Aug;42(1):39-50. An in vitro “gliding assay” shows that axoplasm can be used to cause microtubules to move on slides in an ATPdependent manner Ron Vale lab Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Vale RD, Reese TS, Sheetz MP. Cell. 1985 Aug;42(1):39-50. An in vitro “gliding assay” shows that axoplasm can be used to cause microtubules to move on slides in an ATPdependent manner Ron Vale lab Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Vale RD, Reese TS, Sheetz MP. Cell....
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.

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