Lecture 8--intracellular transport

Vale rd reese ts sheetz mp cell 1985 aug42139 50 an

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Unformatted text preview: 1985 Aug;42(1):39-50. An in vitro “gliding assay” shows that axoplasm can be used to cause microtubules to move on slides in an ATPdependent manner Ron Vale lab Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Vale RD, Reese TS, Sheetz MP. Cell. 1985 Aug;42(1):39-50. Movement of vesicles in the assay requires ATP and the soluble fraction of axoplasm Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. Vale RD, Schnapp BJ, Reese TS, Sheetz MP. Cell. 1985 Mar;40(3):559-69. Purification of the axoplasmic factor that supports axonal transport on microtubules Step 1: Mix AMP-PNP + Microtubules + Optic lobe extract: Microtubules stick to factor, but no movement Step 2: Isolate microtubules with bound factor Step 3: Release factor by adding ATP Step 4: Run protein on a gel filtration column Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Vale RD, Reese TS, Sheetz MP. Cell....
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