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Unformatted text preview: 1985 Aug;42(1):39-50. Purification of the axoplasmic factor that supports
axonal transport on microtubules Kinesin! Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
Vale RD, Reese TS, Sheetz MP. Cell. 1985 Aug;42(1):39-50. Movement on Microtubules Requires
• Progression of organelles along axons requires microtubules and the
motor proteins: kinesin and dynein. Other processes dependent on motor proteins:
• Transport of vesicles for exocytosis/endocytosis or between the
endoplasmic reticulum and Golgi
• Extension of the endoplasmic reticulum
• Integrity and reassembly of the Golgi apparatus A single microtubule can support bidirectional movement Single microtubules from squid axoplasm support bidirectional movement of organelles. Schnapp
BJ, Vale RD, Sheetz MP, Reese TS. Cell. 1985 Feb;40(2):455-62. Dyneins and kinesins travel in opposite
directions and move along individual
protofilaments Kinesin-14 is the exception to the rule: It travels
towards the - end Kinesin 14 Figure 16-63 Molecular Biology of the Cell (© Garland Science 2008) Kinesin
Tetramer: Two heavy chains and two light chains, ~380kD
1. Large globular head: Binds microtub...
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