Lecture 8--intracellular transport

Functions not well known microtubules assemble from

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Unformatted text preview: own Microtubules assemble from organizing centers Pericentriolar material. Each centrosome has 2 centrioles Despite the amorphous appearance, the pericentriolar material of an MTOC is an ordered lattice that contains many proteins necessary for the assembly of microtubules. Microtubules assemble from organizing centers MTOC; Ab against centrosomal protein EM; centrosome (colored red) has two centrioles. PC is pericentriolar material. Microtubule growth from the MTOC can be visualized with EB-GFP fusions EB3 binds to microtubule + ends. Tagging it allows you to watch them grow. Microtubule growth from the MTOC can be visualized with EB-GFP fusions end binding prtein fused to GFP end of every microtubule is what you see as soon as you have live fusion what do you want to do with it? you c make a movie. gamma tubulin is where the microtubules branch from in the cell. microtubules extremely well organized. EB3 binds to microtubule + ends. Tagging it allows you to watch them grow. An MTOC can form without centro...
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.

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