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Unformatted text preview: own Microtubules assemble from organizing centers Pericentriolar
material. Each centrosome has 2 centrioles Despite the amorphous appearance, the pericentriolar material of an MTOC
is an ordered lattice that contains many proteins necessary for the assembly
of microtubules. Microtubules assemble from organizing centers
MTOC; Ab against centrosomal protein EM; centrosome (colored red) has two
centrioles. PC is pericentriolar material. Microtubule growth from the MTOC can be
visualized with EB-GFP fusions EB3 binds to microtubule + ends. Tagging it allows you to watch them grow. Microtubule growth from the MTOC can be
visualized with EB-GFP fusions
end binding prtein fused to
end of every microtubule is
what you see
as soon as you have live
fusion what do you want to do
with it? you c make a movie.
gamma tubulin is where the
microtubules branch from in
microtubules extremely well
organized. EB3 binds to microtubule + ends. Tagging it allows you to watch them grow. An MTOC can form without centro...
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.
- Winter '08