Lecture 1--microscopy

0204 0206 midterm i lectures 1 7 papers 1 2 the

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Unformatted text preview: ns Review for Midterm 5. 02/04 02/06 Midterm I (Lectures 1-7, Papers 1-2) The Cytoskeleton Paper 3: Dupont et al. 6. 02/11 02/13 Cytoskeleton/Cell Motility Cell Motility and the Actin Cytoskeleton Paper 4: Hong et al. 7. 02/18 02/20 Intracellular transport on Microtubules Protein synthesis: ER formation and function Review for Midterm II 8. 02/25 02/27 Protein synthesis: ER formation and function Midterm II (Lectures 8-13, Papers 3-4) Paper 5: Patterson et al. 9. 03/04 03/06 Protein trafficking Protein trafficking Paper 6: van der Zand et al. 10. 03/11 03/13 In class quiz/Lysosomes and Peroxisomes Mitochondrial quality control Review for Final Final: Friday March 21, 11:30 am-1:30 pm Text book reading assignments Lecture 6th edition pages 7th edition pages Microscopy 715 - 726; 729 - 730 731 - 733 732 - 743; 749 - 751 Cell Membranes 117 - 141 120 - 146 Cell Signaling 605 - 612; 614 - 616; 623 - 630; 638 - 640 618 - 625; 627 - 629 636 - 644 ECM and Integrins 230 - 245 236 - 250 Cell-cell Interactions 245 - 258 250 - 264 Cytoskeleton 318 - 326; 347 - 353 330 - 333; 354 - 360 Motility and Migration 354 - 357; 365 - 375 360 - 364; 371 - 382 Intracellular Transport 328 - 339 334 - 345 Protein Synthesis and ER 264 - 283 270 - 289 Trafficking 284 - 297; 301 - 309 290 - 303; 308 - 315 Lysosomes/Peroxisomes 297 - 299; 200 - 201; 309 303 - 305; 206 - 207; 316 Mitochondria 173 - 183; 192 - 200 178 - 183; 199 - 206 Lecture 1 Microscopy: An essential tool in cell biology Microscopy: 1. Cells can be studies in vitro, in vivo, or ex vivo 2. Cellular and molecular scale taking place outside of the cell 3. Light microscopy 4. Fluorescence microscopy and methods for labeling cells with fluorophores 5. Confocal and multiphoton microscopy 6. Super-resolution microscopy 7. Electron microscopy Cell Biology is the study of the structure and function of cells. Cells are the basic structural units of life, since all organisms consist of one or more cells. Differentiated cells vary morphologically and molecularly but are all descended from one fertilized egg and contain the gene expression makes them same DNA different; but dna does not c hange Skin Brain cells Blood Muscle Different cell types have the same organelles but different functions electron microgrqaphs right: fat cell left: secretory cells (black vesicles with digestive enzymes) cells rrsponsive to external environment and can change Cells can be studied in culture or in live tissues red= skin; gree= neurons cultured fibroblasts grwon in a dish zebrafish embryo Tissue culture can be divided into two different c ells Fibroblasts cell i connective tissue that produces collagen and other fibers Cancer cells confluenct and touching the neighbors, they inhibit eachothers grown (contact inhibition)...
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This note was uploaded on 02/23/2014 for the course MCDB 165A taught by Professor Iruela-arispe during the Winter '08 term at UCLA.

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