Ex intron 5 splice site splice donor caagguagagu ex 3

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Unformatted text preview: x of proteins then catalyzes the addition of the poly-A tail In this way, the 3’ end of the message is created Splicing • Eukaryotic transcripts must be spliced to remove intronic sequences • Some introns are self-splicing - the RNA itself has autocatalytic properties (ribozyme) remove its pwn introns • Others require additional cellular factors - the spliceosome Consensus Splice Sequences If you were building an organism in your basement, how/where would you mark introns? ex intron 5’ splice site “splice donor”: C/AAGGUA/GAGU ex 3’ splice site “splice acceptor”: CAGG intro Plus one “A” 18-40 nucleotides upstream of the 3’ site (in the intron) (the “branch point) (Green = intron sequences; gray = exon sequences) Splicing is a two-step process transesterification splicesosome intron 1. The branch point attacks the splice donor site, leading to a transester...
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This document was uploaded on 02/25/2014.

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