Lecture.14.Fall.2013

Ex intron 5 splice site splice donor caagguagagu ex 3

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: x of proteins then catalyzes the addition of the poly-A tail In this way, the 3’ end of the message is created Splicing • Eukaryotic transcripts must be spliced to remove intronic sequences • Some introns are self-splicing - the RNA itself has autocatalytic properties (ribozyme) remove its pwn introns • Others require additional cellular factors - the spliceosome Consensus Splice Sequences If you were building an organism in your basement, how/where would you mark introns? ex intron 5’ splice site “splice donor”: C/AAGGUA/GAGU ex 3’ splice site “splice acceptor”: CAGG intro Plus one “A” 18-40 nucleotides upstream of the 3’ site (in the intron) (the “branch point) (Green = intron sequences; gray = exon sequences) Splicing is a two-step process transesterification splicesosome intron 1. The branch point attacks the splice donor site, leading to a transester...
View Full Document

This document was uploaded on 02/25/2014.

Ask a homework question - tutors are online