Lecture.12.Fall.2013

Alu is named after the restriction enzyme alui which

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Unformatted text preview: TRANSFER TEs in humans: Alu REPLICATIVE: TRANSPOSED INTO EVERY HUMAN CHROM Human metaphase chromosomes: Alu Sequence is stained green, DNA is red. • Alu is named after the restriction enzyme AluI, which cleaves the element in two • Each human cell contains >1,000,000 copies. TEs in humans: Alu Human metaphase chromosomes: Alu Sequence is stained green, DNA is red. • Creates short flanking direct repeats when it inserts. • Belongs to a class of repetitive sequences in mammals called SINEs, for Short Interspersed Elements TYPE OF REPLICATIVE DNA Sleeping Beauty Question 4 • Do you think when Barbara McClintock began her studies of maize genetics that she knew she would discover TEs, and that they might be developed for use in gene therapy? • ANSWER: Probably not. Sleeping Beauty Question 4b • Why do you think she studied it? • ANSWER: Because it was interesting. • Why do scientists do basic research today? • ANSWER: Because it’s interesting. • And applied research directly aimed at curing diseases strongly relies on previous basic research. • Where would the gene therapy experiments be without McClintock’s basic science? Transposable Elements Replication October 7, 2013 • DNA Replication (Chapter 12) • Three models: Conservative, Dispersive, and Semiconservative • Meselson and Stahl’s experiment to distinguish among them • Modes of replication in bacteria and eukaryotes • Fidelity o...
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