Lecture.12.Fall.2013

Dna transposons require an enzyme called transposase

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Unformatted text preview: one site to another without replicating itself. • DNA transposons require an enzyme called transposase catalyze movement of transposon • • • Figure 11.14 usually encoded by the TE. Transposase cleaves TE and target DNA. The TE is ligated to target DNA by the cell’s enzymes. cut and paste, no replication Class 2. DNA Transposons Replicative Transposition This is more complicated than I want to deal with in this course Your book has a somewhat confusing graphic; feel free to look it over You won’t be tested on the mechanism, but you must know that many DNA transposons replicate themselves during transposition Thus, their number increases with time Development of the Sleeping Beauty Transposon: Question 2 In question 1, we identified the TE from the genome. Now we do a search and confirm that it encodes a transposase: Transposase ! Can you tell from the gene it encodes what class of TE is it? ! What gene might it encode if it was the other class? class 2 reverse transcriptase Development of the Sleeping Beauty Transposon: Question 2 mutagenic: casue new mutat...
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This document was uploaded on 02/25/2014.

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