Coli grown in this rare isotope meselson and stahls

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Unformatted text preview: ensity gradient centrifugation to differentiate between: • “Light” 14N-containing DNA made by E. coli grown in a normal nitrogen source. Meselson and Stahl’s experiment • “Heavy” 15N-containing DNA made by E. coli grown in this rare isotope Meselson and Stahl’s experiment All DNA Replication is Semiconservative • Semiconservative replication: • During replication, each original strand of DNA serves as the template for synthesis of a new strand. • The original DNA molecule is half (semi) conserved during replication. • All DNA in all known organisms replicates using a semiconservative mechanism • However, there are different modes of replication based on the type of template. Modes of Replication Theta replication (small circular molecules) Rolling-circle replication (not covering this in detail) Multiple-origin replication for large, linear genomes Modes of Replication: Theta (θ) replication for circular DNA BACTERIA SINGLE ORIGIN; ORIGIN=WHERE REPK BEGINS Modes of repli...
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This document was uploaded on 02/25/2014.

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