Facultative catalase salt tolerant 15 and ph

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Unformatted text preview: atalase +)/salt tolerant (15%) and pH tolerant(5-6) HIGHLY SALT TOLERANT, CAN GROW IN MANNITOL SALT AGAR S. aureus- Non pathogenic & pathogenic (coagulase) Proteases/coagulase/hemolysins/leukocidin/exfolliative toxin/penicillinase/exotoxins: toxic shock toxin & enterotoxin/skin lesions (carbucles/abcesses) S. AUREUS IS HEMOLYTIC. CAN KILL WHITE BLOOD CELLS, MOST ARE NOW RESISTANT TO PENICILLIN, TYPICAL ENTEROTOXIN SYMPTOMS, FAST REPLICATION RATE SYMPTOMS CAN APPEAR WITHIN HOURS. LARGE VOLUME OF FOOD CANNOT BE COOLED PROPERLY AND QUICKLY ENOUGH, THUS, S. AUREUS CAN REPRODUCE QUICKLY. CAN ALSO CAUSE SKIN LESIONS (PIMPLE, TURBUCLE) S. epidermidis- Colonizes man & animals Ferments glucose, but not mannitol EPIDERMIDIS IS THE BENIGN COUSIN OF S. AUREUS, IT ONLY FERMENTS GLUCOSE BUT NOT MANNITOL ONE OF THE VIRULENCE FACTORS OF THIS ORGANISM IS THAT IT CAN CLOT FIBRIN USING MATERIAL FOUND IN YOUR OWN BLOOD. SOME STAPH. AUREUS ARE COAGULASE POSITIVE SOME ARE NOT. Staphylococcal cell arrangement GRAPE LIKE CLUSTER IN RANDOM ARRANGEMENT Mannitol Salt agar Coagulase test COAGULASE NEGATIVE COAGULASE POSITIVE: FIBRIN IN TUBE WILL CLOT (VIRULENCE FACTOR). THIS IS A MECHANISM THAT ALLOWS STAPHYLOCOCCUS TO WALL ITSELF OFF MRSA: METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS, ALL ARE RESISTANT TO PENICILLIN, BUT ARE NOW RESISTANT TO METHICILLIN Gram positive coccus ? Catalase test Text HOW CAN YOU SEPARATE BETWEEN MICROCOCCUS AND STAPHYLOCOCCUS AND OTHER COCCI =PERFORM A CATALASE TEST CATALASE IS REQUIRED BY AEROBESTOGET RID OF RADICAL OXIDES NO BUBBLES= AEROTOLERANT ANAEROBES ; THEY DO NOT HAVE A CYTOCHROME SYSTEM AND MUST RESPIRE ALL THE TIME. ~aerobes or facultative anaerobes (Staphylococcus sp. and Micrococcus sp.) CATALASE POSTIVE= STAPH.MICRO ~aerotolerant anaerobes (lactic acid bacteria) PRODUCE LACTIC ACID BY FERMENTATION 30:43 Lactic acid bacteria “ONE ROD” Produce lactic acid by fermentation/no cytochromes/fastidious IF IT IS A COCCUS, THERE ARE HETERO AND HOMOFERMENTERS Lactic acid bacteria Cocci Bacilli* Lactobacillus sp PRODUCES MORE THAN ONE ACID END PRODUCT Homofermenters Heterofermenters Leuconostoc sp PRODUCE SINGLE TYPE OF ACID END PRODUCT PRODUCT =LACTIC ACID LEUCONOSTIC CAN SPOIL VEGETABLE SUCH AS CELERY USE CELL ARRANGEMENT TO DIFFERENTIATE BETWEEN HOMOFERMENTERS Streptococcal Streptococcus sp Tetrad Pediococcus sp Text STREPTOCOCCUS IS PUT IT CATEGORIES BASED ON CELL WALL AND HEMOLYTIC PATTERN (BETA=CLEAR LYSIS OF RBC, ALPHA AND GAMMA...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State.

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