R groups are long aliphatic chains hydrophobic waxy

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Unformatted text preview: ATIC CHAINS, HYDROPHOBIC; “WAXY” SUBSTANCE THAT SURROUNDS THE CELL AND PREVENT COMPLETE CONTACT WITH AGAR PLATE. NUTRIENTS MUST DIFFUSE AND PENETRATE HYDROPHOBIC LAYER, SLOW GROWING AS A RESULT. Mycolic acid 1. Acid Fast Stain a. Flood the slide with Ziehl's carbol fuchsin CLEAR AT START and steam directly for 8 minutes. BOTH PINK b. Rinse with distilled water, blot dry. T c. Gently decolorize by rapidly dipping the slide into a Coplin jar filled with 1% acid-alcohol. DESTAIN PINK AND CLEAR d. Rinse with distilled water. COUNTERSTAIN PINK AND BLUE e. Flood slide with methylene blue for 30-60 seconds. f. Rise, blot dry and observe using the oil immersion objective. YOU TYPICALLY END UP WITH BLUE STAINED CELL (METHYLENE BLUE). RUDIMENTARY BRANCHES ARE FROM TUBERCULOSIS (ACID FAST MYCOLIC ACID POSITIVE) 11/20 9:39 ENDEMIC TO ASIA YOU NEVER REALLY GET RID OF TUBERCULOSIS, IF THERE IS A STIMULUS (SUCH AS OLD AGE OR STRESS, THE BACTERIUM GETS THE UPPER HAND). THIS LEADS TO THE DEGENERATION OF THE LUNGS. TUBERCLE IS A DEGENERATION OF THE LUNG TISSUE. Acid fast-stained sputum Mycobacterium sp. PERSISTENT INFECTION IN LUNG TISSUE. M. tuberculosis - Produces tubercle (focus of infection) Diagnosis-->tuberculin skin test SPUTUM SAMPLE, XRAY SKIN TEST: SERODIAGNOSIS—YOU ARE DETECTING ANTIBODIES MADE IN RESPONSE TO A SPECIFIC INFECTION. WITH TUBERCULOSIS, THE ORGANISM DOES NOT ALWAYS MAKE ANTIBODIES, BUT IT DOES CREATE AN IMMUNE RESPONSE. TUBERCULOSIS DOES MAKE IMMUNE CELLS WITH SPECIFIC RECEPTORS. ANTIGENS ARE PRESENT ON SURFACE OF THE CELL. LOOK FOR SPECIFIC MYCOBACTERIUM TUBERCULOSIS T LYMPHOCYTES MADE BY OWN IMMUNE SYSTEM. M. leprae- nervous tissue M. tuberculosis INTRADERMAL MATERIAL INJECTED INTO SKIN TO DETECT ANTIGEN, IF ANTIGEN IS PRESENT, THEN T LYMPHOCYTES WILL SECRETE LYMPHOKINES (ACTIVATING CELLS) THIS RECRUITS AND ACTIVATES CELLS. LYMPHOCYTES ARE ATTRACTED TO SITE AND A HARD LUMP=LYMPHOKINES M. LEPRAE = LEPROSY; HANSEN’S DISEASE. AFFINITY FOR NERVOUS TISSUE SLO...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State University .

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