F13 17 Viruses and Prions

Cell culture flask of cells grown on flat surface

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Unformatted text preview: IRUSES CAN INFECT. CELL CULTURE: FLASK OF CELLS GROWN ON FLAT SURFACE. LOOK FOR CYTOPATHIC EFFECTS SMALL POX: ERADICATED BY VACCINE BUT HAS POTENTIAL FOR USE AS BIOLOGICAL WARFARE AGENT. VIRUS IS STILL ALIVE IN LABS. T POLIO: WATER CONTAMINATED VIRUS, AFFECTS CENTRAL NERVOUS SYSTEM AND LEADS TO PARALYSIS. ALL IMMUNIZED. THERE HAVE BEEN NEW POLIO OUTBREAKS IN WAR ZONES. Eradication? PRIONS PRIONS ARE BASICALLY PROTEIN. ARE THEY ALIVE? PRIONS ARE TRANSMISSIBLE BETWEEN PEOPLE. “TSE” TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHY, MANY VARIANTS DISCOVERED BY GAJDUSEK; FOUND MAINLY IN WOMEN AND CHILDREN DUE TO INFECTED SCRAPS BEING EATEN (BRAIN) OF DEAD HUMANS. AFFECTS MENTAL COGNITION, LOSS OF MOTOR SKILLS AND CAUSES A WASTING DISEASE. THIS PROTEIN IS FOUND IN NORMAL CELLS, SO WHY IS DISEASE SEEN SOME BUT NOT OTHERS? THIS IS DUE TO A GENETIC DISEASE THAT PREDISPOSES PEOPLE TO THIS DISEASE DUE TO CANNIBALISTIC RITUAL. THERE IS A MUTATION THAT PREDISPOSES OUR PROTEINS TO BECOME PRIONS 19:25 12/4 CAN BE TRANSMITTED BETWEEN PEOPLE • Transmissible Spongiform Encephalopathy (TSE) Carl Gajdusek Scrapie/Kuru/Creutzfeldt-Jacob disease/Gerstman-StrausslerScheinker (GSS) syndrome/Bovine spongiform encephalopathy • PrP Prion protein: no nucleic acid (DNA or RNA)/ ’replicates’/infectious • PrPC: Found in normal cells • PrPSC: Found in TSE cells only/ cytoplasmic vesicles (amyloid protein) • PrPSC converts PrPC to PrPSC : ‘Replication’ ?? • Genetic link: Mutations in PRNP gene which predispose cells to convert PrPC to PrPSC. THEY ONLY HAVE PROTEIN GENE THAT HAS A TENDENCY TO BE CONVERTED INTO PRIONS PRIONS CAN CROSS SPECIES LINES PROTEIN THAT WE NORMALLY HAVE HAS HELICAL FORM, IN PRION DISEASE, A CONFORMATIONAL SHIFT OCCURS, AND A SINGLE BASE MUTATION OCCURS IN CDJ THAT MA...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State.

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