F13 17 Viruses and Prions

T antigenic shift one way that a virus evolve over if

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Unformatted text preview: CAN KILL RAPIDLY. T ANTIGENIC SHIFT: ONE WAY THAT A VIRUS EVOLVE OVER IF NEW GENETIC ELEMENTS ARE PACKED INTO A NEW VIRUS, ITS ANTIGENIC SHIFT ANTIGENIC DRIFT: VIRUS IS HIGHLY MUTATED AND CONTINUOUSLY MAKE NEW GENETIC COMPOSITION DUE TO MUTATIONS ALONE IF ANTIGENS CHANGES AS A RESULT, THAN IT IS ANTIGENIC SHIFT. VIRUSES MUST BE CULTIVATED USING LIVING CELLS (PLAQUE ASSAY) PLAQUES (VIRUSES) CAN BE CULTURED USING PLAQUE PURIFICATION (LIKE STREAK PLATE) BUT LIVING CELLS ARE ALWAYS REQUIRED. Growing viruses: plaques & plaque-forming units (PFU) burst size (multiplicity of infection) Text Text GENOME IS REPLICATED, STILL NO VIRUS Phage ‘growth curve’ EARLY ENZ= REVERSE TRANSCRIPTASE, ECT. LOOKING AT PLAQUE FORMING UNITS OVER TIME. -ATTACHMENT OF VIRUS TO HOST CELL, THERE ARE NO REPLICATION, VIRUS IS OUTSIDE HOST CELLS -ONCE VIRUS INJECTS GENOME INTO HOST, THERE ARE EARLY ENZYMES PRODUCED (REVERSE TRANSCRIPTASE, ENZ FOR VIRAL CAPSID SYNTH) -THEN THERE IS A PERIOD WHERE GENOME IS REPLICATED, STILL NO INTACT VIRONS/INFECTIVE VIRUSES -PROTEIN COAT ASSEMBLY, STILL NO INFECTIVE VIRUSES -PERIOD OF TIME FROM INJECTION OF GENOME AND BEGINNING OF INTACT VIRUS=ECLIPSE PERIOD -ONE CAPSID IS PRODUCE, GENOME IS REPLICATED -ONCE ASSEMBLED, INTACT VIRONS CAN BE SEEN AFTER LYSIS INTACT VIRULENCE CAN BE OBSERVED PLAQUE MAMMALIAN CELL Culturing viruses: whole organism, embryonated egg, & cell culture EMBYONATED EGGS: CHICK EGGS ARE A POPULAR PLACE FOR ANIMAL VIRUSES TO BE GROWN CHORIOALLONTOIC MEMBRANE IS A POPULAR PLACE BECAUSE IT HAS A LOT OF UNDIFFERENTIATED TISSUE THAT V...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State.

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