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Unformatted text preview: G LIGHT SPECTRUM. 40 MIN Fluorescence Microscope
BLOCK YOU WOULD JUST SEE WHITE LIGHT
BECAUSE UV LIGHT HAS BEEN
BLOCKED, NOT EXCITATION OF FLUOR
DYE WHITE LIGHT
BLOCK WHITE LIGHT, ALLOW FLUOR LIGHT,
WE SEE FLUOR BUT NOT SEE WHITE LIGHT NO BLOCK, WE SEE ALL. Can you guess?
Neither Confocal Microscopy
• inverted microscope with
- a laser light source
- the laser scanning head
- an automatic focusing stage
- connected to a monitor and
USE FLUOR AS WELL, BUT HAS LASER
INSTEAD OF UV LIGHT SOURCE. SCANNING HEAD LIKE A TV SO IT
CAPTURES CERTAIN FOCAL PLANE IN EACH PASS. CONNECTED TO COMPUTER BECAUSE EVERYTIME
LASER HEAD MAKES A PASS, IT CONTRUCTS A 3D IMAGE OF THE SPECIMEN. WHAT CAN IT DO FOR YOU? PASSES THRU SINGLE CELL, COMPUTER RECONSTRUCTS 3D IMAGE, SEE CELL ATTACHMENT
ECT. Confocal Microscopy
• The CM filters out the out-of-focus light from the above and below
point in the focus of the object by a pinhole.
• Lasers are used because they produce extremely bright light at spe...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State University .
- Fall '14