Associ with it looking for specific aspect of cell

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ODY TAG WITH FLUOR. ASSOCI WITH IT, LOOKING FOR SPECIFIC ASPECT OF CELL THAT IS PRESENT USING THE TAG. (GENE SEQ) CAN ALSO USE TO DET. VIABILITY OF CELL Fluorescence Microscope low / high E high / low E low / high E high / low E HOW DOES IT WORK: SHINE HIGH NRG LIGHT ON SPECIMEN WITH FLUOR TAG WILL EMT PHOTONS AND WE SEE THE PHOTONS, WHEN PHOTONS ARE EMITTED, ENERGY IS LOST AND WE CAN SEE THE LOSS IN VISIBLE LIGHT RANGE. LIGHT CAN BE SEPARATED BY WAVELENGTH. DEPENDING ON WL, WE CAN PERCIEVE LIGHT DEPENDING ON PARTICULAR COLOR WE SEE PLANTS AS GREEN BECAUSE THE PIGMENTS OF PLANTS ABSORB RED LIGHT. THERE ARE WL BELOW VISIBLE SPECTRUM, LOW IN WAVELENGTH, HIGH IN NRG (UV RAY) INFRARED: HGH WAVELENGTH, LOW NRG Fluorescence Microscope COMPONENTS: UV LIGHT SOURCE (MAKES HIGH NRG LIGHT NEEDED) ,DICHROIC MIRROR "FILTERS" THAT CONTROL LIGHT THAT GOES THRU OBSERVE HERE 550 NM EMITS PHOTON @ 550 NM FLUOROISOTHICYNATE: EMITS LIGHT IN VISIBLE RANGE. ONLY VISIBLE LIGHT GOES THRU, HIGH NRG ARE REFLECYED BACK, IMPORTANT TO NOT DAMAGE EYES AND WE WANT TO SEE LOW NR...
View Full Document

This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State.

Ask a homework question - tutors are online