The computer allows the system to scan sequential

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Unformatted text preview: cific wavelengths. • The computer allows the system to scan sequential planes in the Zdirection apart from X and Y direction,store and create overlays to create 3-D images. 3D reconstruction in CM 40 optical sections of the cell Reconstructed 3-D model Electron Microscope • Illuminating energy comes from electrons instead of light GOOD FOR SMALL DETAIL, GO BELOW 0.2 MICRONS. SAME PRINCIPLE, NRG SOURCE IS ELECTRONS (ELC BEAM) SHONE ONTO SPECIMEN ELECTROMAGNET LENS • Lenses are electromagnets instead of glass. ACHIEVE GOOD RESOLUTION AT HIGH MAGNIFICATION Magnification (2000X plus) BECAUSE THEYRE NOT LIMITED TO WHITE LIGHT AS ILLUM. NRG Contrast (Stain with electron dense material, e.g. gold) IF ELEC HITS DENSE MATERIAL, IT DOESNT Resolution (Resolves in nm range) PASS THRU. IF IT PASSES THRU=CONTRAST -------> RP = /NA x 2 (Electron microscope: RP = small / large -->RP small) RESOLUTION IN NANOMETER RANGE IN LIGHT MICROSCOPE, WE USE 550 NM AS STANDARD, IN ELEC. THE WAVELENGTH IS SMALL AND VERY HIGH NRG • Scanning electron microscope (SEM) NUMERICAL APERATURE: NO RELAINCE ON GLASS, ELECTROMAGNETS USED TO DUE TO HIGH NUMERICAL APERATURE. • Transmission electron microscope (TEM) 2 TYPES: SCANNING AND TRANSMISSION...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State University .

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