F13 4 Invest and Media

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Unformatted text preview: HER ORGANISMS GROWING WITH IT 1. Discovered the causative agent of tuberculosis. 2. Discovered the causative agent of cholera. 3. Developed pure culture techniques using colonies on solid medium. KOCH INSTITUTE ALSO CREATED. DEVELOPED TECHNIQUES BASED ON PURE CULTURE FIRST PERSON TO GROW MO'S ON SOLID AGAR MEDIUM IDEA OF AGAR MEDIUM: PUT LIQUID MEDIUM ONTO SOLID MEDIUM, IF A CELL LANDS ON SLOID MEDIA, IT CAN MULTIPLY AND PRODUCE MANY CELLS UNTIL IT BECOMES VISIBLE TO THE NAMED EYE (COLONY) IF YOU HAVE A COLONY, THESE ARE ALL CLONES, ALL PROGENY FROM SINGLE CELLS GELATIN WAS USED AS SOLIDIFYING AGENT, BUT GELATIN IS FREQUENTLY MELTS EASILY AND CAN BE USED AS A NITROGEN SOURCE BY MO'S AGAR COMES FROM SEAWEED, DEVELOPED BY AMERICAN WOMAN IN KOCH'S LAB Robert Koch INVENTED PURE CULTURE, SOLID AGAR MEDIA, AND PETRI DISH Anthraxbroth culture Many MO's will grow on solid medium and form colonies. Each colony represents the growth and repeated replication of a single original cell.CLONES Solid medium-->single colony isolation-->pure culture Agar (melts at 95°C/solidifies at 45°C) SOLID AT ABOUT 55 Petri dish DEVELOPED BY ITALIAN IN KOCHS LAB Pure culture (mixed culture/contaminated culture) AGAR: IS A POLYMER OF D-GALACTOSE, HAS SPECIFIC MELTING AND SOLIDIFYING PROPERTIES THIS OXYGEN LINK CAUSES PROPERTIES OF AGAR Microbial culture • Terms: Broth, agar, complex media, synthetic media, selective media, differential media, differential/selective • Sterility: Gas/filtration/heat Autoclave Sterile/aseptic PURE CULTURE: CULTURE WITH ONLY ONE ORGANISM MIXED CULTURE: CULTURE WITH MORE THAN ONE ORGANISM CONTAMINATED CULTURE: PURE CULTURE THAT HAS ACQUIRED ANOTHER MO'S DUE TO AIR EXPOSURE, PEPTONES: TAKE BEEF MUSCLE TISSUE, PLACE IN WATER BATH, HEAT AND EXTRACT NUTRIENTS FRO...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State University .

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