F13 4 Invest and Media

Tissie with enzyme results in peptone a mixture of

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Unformatted text preview: M MUSCLE TISSUE AND ALSO DIGEST MUSCLE TISSIE WITH ENZYME; RESULTS IN PEPTONE, A MIXTURE OF PROTEINS, PEPTIDES, AMINO ACIDS, Nutrient broth: Minimal glucose medium: LIQUID Peptone Beef extract Water 5.0 g 3.0 g 1000 ml BEEF EXTRACT: PLACE MUSCLE YOU CANNOT DESCRIBE TISSUE IN WARM WATER, SOME CHEMICAL FORMULA OF NUTRIENTS THAT ARE PEPTONE DEGRADED BY ENZYMES RESULTS IN GENTLE EXTRACT OF MUSCLE TISSUE. COMPLEX MEDIA: CANNOT BE DEFINED Nutrient agar: Peptone Beef extract Agar Water Glucose NH4H2PO3 NaCl MgSO4 K2HPO4 Water SYNTHETIC/DEFINED 5.0 g 1.0 g 5.0 g 0.2 g 1.0 g 1000 ml EVERY COMPONENT KNOWN: DEFINED/SYNTHETIC SOLID 5.0 g 3.0 g 15.0 g 1000 ml carbon & nitrogen MAIN SOURCE HAS C/N, BUT growth factors ALSO SOURCE OF GF MAIN solidifying agent solvent SOLID AGAR MEDIA ALLOWS EASY SEPARATION OF SINGLE COLONIES GROWTH FACTORS: USED BY MO'S DIFFERENTIA MEDIA: CAN TELL DIFFERENCE BETWEEN BIOCHEMICAL REACTIONS MANNITOL SALT AGAR: LIKE NUTRIENT AGAR, HAS PHENOL RED, MANNITOL (SUGAR-ALCOHOL) AND SALT; PHENOL RED CREATES RED APPEARANCE. THREE POSSIBILITY OF GROWTH 1. NO GROWTH: 7.5 % SALT RESTRICTS GROWTH OF MO 2.SOME ORGS GROW BUT NOT CHANGE IN MEDIA 3. GROWTH AFFECTS THE MEDIA, YELLOW COLOR CHANGE YELLOW COLOR INDICATES MANNITOL FERMENTATION. MANNITOL CONVERTED INTO ACID RED PRODUCT.TELLS YOU THAT IT FERMENTS CARBOHYDRATE • Blood agar BASICALLY, BLOOD AGAR IS LIKE NUTRIENT AGAR BUT WITH ADDED SHEEPS RBC (DEFIBRINATED-FIBRIN REMOVED TO PREVENT CLOTTING). CLEAR ZONE: DUE TO CELL HAVING HEMOLYSINS/LIPASES THAT DEGRADE THE RBC, THE RBC DISPERSE CREATING CLEAR ZONE DIFFERENTIAL AND SELECTIVE • Mannitol Salt Agar...
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State.

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