If you come immediately down at normal pressure the

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Unformatted text preview: ID. IF YOU COME IMMEDIATELY DOWN AT NORMAL PRESSURE, THE GAS WANTS TO COME OUT AND IT BOILS OVER SOME ADDITIVE/GROWTH FACTORS REQ'D BY ORGANISMS ARE DESTROYED BY HEAT, SO IT CANNOT BE AUTOCLAVED. IT MUST BE STERILIZED COLD. COLD FILTRATION: STERILIZE MATERIAL IN AUTOCLAVE ADD NON AUTOCLAVEABLE MATERIAL THATS STERILIZED USE COLD STERILIZATION GAS PRESSURE APPLIED OUTSIDE PORES ARE UNIFORM SIZED MILLIPORE CORP. PRODUCED MEMBRANE WITH SPECIFIC SIZE ANY CELLULAR MATERIAL LARGER THAN .45 MICRONS GETS CAUGHT IN FILTER EVERYTHING ELSE GOES THRU AVAILABLE PORE SIZES: .45 MICRONS IS MOST COMMON MOST TISSUE CULTURES ARE STERILIZED USING COLD FILTRATION BECAUSE THEY HAVE HEAT LABILE COMPONENTS DISADVANTAGE: CANT FILTER GLASSWARE GAS FILTRATION USE TO STERILIZE LARGE, HEAT SENSITIVE EQUPIMENT USES FOR ETO TO STERILIZE TUBES, PLATES, ECT Microbial culture • Terms: Broth, agar, complex media, synthetic media, selective media, differential media, differential/selective • Sterility: Gas/filtration/heat Autoclave Sterile/aseptic ASEPTIC: NOT CONTAMINTED YOU CANNOT HAVE A STERILE CULTURE, OTHERWISE THERE WOULDNT BE ANYTHING IN IT assimilatory: build up of cell material DISSIMILATORY: TAKE CHEMICAL APART, USUALLY FOR PURPOSES OF ENERGY Assimilatory/Dissimilatory metabolism CELLS MUST DO BOTH DOING BOTH ASS/DISS ALLOWS CELL TO OPTIMIZE MICROBIAL GROWTH (TO DOMINATE ENVIRNMENT, be motile, metabolize, GAIN ACCESS TO MOST RESOURCES, AND OUTCOMPETE OTHER ORGANISMS Nutritional classi...
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