In minimal glucose medium phosphate buffer system

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Unformatted text preview: . IN MINIMAL GLUCOSE MEDIUM: PHOSPHATE BUFFER SYSTEM Agar POLYSACCHARIDE, MADE OF REPEATING UNITS OF GALACTOSE SOLIDIFYING AGENT, NOT TOXIC. MELTS AT 95, SOLID AT 55, CANT BE METABOLIZED Na silicate + H3PO4 (added slowly)--> solid IF YOU ARE TRYING TO GROW CHEMOAUTOTROPHS (CARBON FROM INORG), THEY WILL NOT GROW BECAUSE THEY ARE INHIBITED FROM GROWING IN PRESENCE OF ORGANIC CARBON. ---NO AGAR! USE SILICATE INSTEAD MIXED WITH PHOSPHORIC ACID; LET SIT OUT---NO ORGANIC CARBON IN IT CAN USE PURE CULTURE TECHNIQUES. AGARLESS! Nutrient agar: Peptone Beef extract Agar Water COMPLEX MEDIUM C&N gf solidifier solvent GROWS A LOT OF CHEMOHETEROTROPHS, WONT GROW CHEMOAUTOTROPHS Chemoautotrophic medium: THUS, USE CHEMOAUTROPH MEDIUM: LET THEM GET CARBON FROM AIR LIKE A PLANT NITROGEN FROM AMMONIUM CHLORIDE ENERGY FROM INORGANIC SOLIDIFIER IS SILICATE AND WATER OXID FOR NRG CO2 /CaCO3 NH4Cl S0/Fe+++/H2S/.. Mg/S/P/… Silicate (glass) Water C N energy minerals solidifier solvent NO AGAR NO AGAR NO AGAR CHEMOAUTO CANT GROW IN CARBON PRESENCE....
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This document was uploaded on 02/25/2014 for the course MICROBIOLO 101 at San Jose State University .

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