F13 8 Taxonomy and Symbiosis Concepts(2)

That cannot grow you can use 16s to identify

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ANNOT GROW, YOU CAN USE 16S TO IDENTIFY sun=radiant energy RICE PLANT: FIXES OXYGEN FROM ATMOSPHERE, IT IS PHOTOAUTOTROPH, MAKES CATALASE Symbiosis Terms & Concepts Microbial Culture & Enrichment CATALASE TESTS FOR OXYGEN BEGGIOTOA: MICROAEROPHILIC, WONT GROW WHERE THERES A LOT OF OXYGEN, NEEDS REDUCED OXYGEN WILL FIND REDUCED OXYGEN MID WAY DOWN PLANT; GETS NRG BY REDUCING HYDROGEN SULFIDE BUT PRODUCES H202 WHICH IS TOXIC FOR THE PLANT. HOWEVER BEGGIOTOA DONT PRODUCE CATALASE. limit of sun penetration THERE ARE PHOTOAUTOTROPHS UP HERE PRODUCING OXYGEN/NRG FROM THE SUN OXYGEN; MORE AT TOP, LESS AT BOTTOM HETEROTROPHS, SCAVENGING OXYGEN, LESS OXYGEN TOWARDS BOTTOM Lab: Winogradsky Unculturable Polymerase Chain Reaction Template=>chromosome of unknown (contains 16s rDNA) Primers=>Conserved, bookend 16s rDNA DNA polymerase=>enzyme ANAEROBE dNTPs=> (ATP, GTP, CTP & TTP) Thermal cycle=>melt->anneal->extend DESULFOVIBRIO; ANAEROBE, USES SO3 AS TERMINAL ELEC ACCEPTOR, RESPIRE ANAEROBICALLY AND REDUCES H2S; H2S IS TOXIC TO RICE PLANT IF WE ARE HEALTHY, IT IMPLIES THAT WE ARE MAINTAINING HOMEOSTASIS (BODY TEMP, N...
View Full Document

Ask a homework question - tutors are online