272_Exp2A_report

Be sure to use the correct number of significant

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Unformatted text preview: the discretion of the grader) will be marked “zero.” Calculations: 1. Calculate the concentration of BSA in each standard sample before it is mixed with the Coomassie blue and complete the table below. Be sure to use the correct number of significant figures for all volumes and concentrations. Show your work for Sample 4. (6 points) Concentration of BSA stock solution:___________________________________ Sample # Vstock BSA, µ L 1 Vtotal, µ L VH2O, µ L [BSA], µ g/mL 2 3 4 5 6 7 8 Error in the micropipet: + __________________ µ L Concentration calculation for sample 4: 2. Calculate the concentration of BSA in the cuvet (after it has been mixed with Coomassie blue) for each sample. Determine the average absorbance for each sample and the uncertainty (standard deviation) in the absorbance for each sample. Complete the table below. Show your work for sample 4. (7 points) Sample # [BSA]cuvet, µ g/mL 1 Avg A Uncertaint...
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This lab report was uploaded on 02/24/2014 for the course CHEM 131 taught by Professor ... during the Spring '08 term at Maryland.

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