272_Exp2A_report

Concentration use the concentration as calculated in

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: y in A 2 3 4 5 6 7 8 A ± uncertainty Concentration of sample 4: Average absorbance calculation for sample 4: Standard deviation calculation for sample 4: 3. Make a Beer’s Law standard calibration plot by plotting the average absorbance vs. concentration. Use the concentration as calculated in question 2. Follow the instructions for making plots as outlined in the lab manual introduction. (6 points) a. Fit the data with a straight line. If the data extend beyond the linear range, do not include the non- linear points. b. Use the standard deviations to include error bars on your absorption measurements. c. Staple your plot to the back of this report. 4. Use the plot to determine the extinction coefficient (ε) for the CBBG- BSA complex. (Note: Typically, the molar extinction coefficient is reported in units of M- 1 cm- 1. Since you don’t have the molar mass of BSA, you will report the extinction coefficient in units of mL...
View Full Document

This lab report was uploaded on 02/24/2014 for the course CHEM 131 taught by Professor ... during the Spring '08 term at Maryland.

Ask a homework question - tutors are online