272_Exp2A_report

Concentration use the concentration as calculated in

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Unformatted text preview: y in A 2 3 4 5 6 7 8 A ± uncertainty Concentration of sample 4: Average absorbance calculation for sample 4: Standard deviation calculation for sample 4: 3. Make a Beer’s Law standard calibration plot by plotting the average absorbance vs. concentration. Use the concentration as calculated in question 2. Follow the instructions for making plots as outlined in the lab manual introduction. (6 points) a. Fit the data with a straight line. If the data extend beyond the linear range, do not include the non- linear points. b. Use the standard deviations to include error bars on your absorption measurements. c. Staple your plot to the back of this report. 4. Use the plot to determine the extinction coefficient (ε) for the CBBG- BSA complex. (Note: Typically, the molar extinction coefficient is reported in units of M- 1 cm- 1. Since you don’t have the molar mass of BSA, you will report the extinction coefficient in units of mL...
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