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Unformatted text preview: icroscopy. Pulse- Chase and Autoradiography was used to discover the pathway of secreEon in the 1950s and 60s RadioacEve proteins labelled in red 3 min 20 min 120 min You will discuss these techniques in tutorial. Fig. 8- 3 from Karp: Cell and Molecular Biology Data from autoradiography autradiographic signal is black squigglies The secretory (sec) mutants were important in dissecEng the proteins involved in secreton Temperature- sensiEve yeast secretory (sec) mutants Temperature- sensiEve mutants are only blocked at higher temperatures. At lower temperatures the protein funcEons normally Lodish et al. 1999, Fig. 17- 14 Secretory mutants of yeast Saccharomyces cerevesiae = budding yeast (makes beer and bread) restricEve temp = 37°C permissive temp = 2...
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