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Fig 8 3 from karp cell and molecular biology data from

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Unformatted text preview: e Cell (© Garland Science 2008) Fig. 15- 27 (15- 28 2nd ediEon): Regulated SecreEon Example: Release of neuotransmilers at synapse Hirsch N P Br. J. Anaesth. 2007;99:132- 138 Regulated SecreEon Example: Release of histamine- containing granules of mast cells Figure 13- 68 Molecular Biology of the Cell (© Garland Science 2008) Regulated secreEon vs. ConsEtuEve secreEon Which is which? pancreaEc cell How can you tell? secreEng digesEve enzymes Lymphocyte secreEng anEbodies Experimental methods used to gather evidence on the Secretory pathway 1.  Autoradiography: Uses radioacEvely labeled macromolecules to locate specific compounds in the cell. 2.  Studying geneHc mutants with mutaEons in key secretory proteins. Example: yeast (Saccharomyces cerevisieae) secretory (sec) mutants. 3.  Secretory proteins geneHcally fused to Green Fluorescent Protein (GFP): labeled proteins can be tracked in live cells using confocal m...
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