Exam 3 Review & Notes

On and recombinaon are required to give plaques in

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Unformatted text preview: nor recombina.on 8. Phage are mixed at high mul.plicity with K cells and plated at low mul.plicity on B cells. For crosses involving two T4 rII- mutants in the following two ques.ons, indicate which of the following is REQUIRED to give plaques (either mutant or wildtype plaques are acceptable). a) Complementa.on b) Recombina.on c) Complementa.on plus recombina.on d) Neither complementa.on nor recombina.on 8. Phage are mixed at high mul.plicity with K cells and plated at low mul.plicity on B cells. The rII locus consists of two genes, A and B. If two muta.ons can complement (one has a muta.on in A and the other in B) then they can infect K cells without prematurely killing the cells since both the A and B proteins are made. From Dr. Zitomer’s lecture notes, page 115: “Infect K12...
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