Explain or illustrate effects of ph on enzyme

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Unformatted text preview: ). Know the complete chymotrypsin reaction mechanism, including the catalytic residues, the catalytic steps, substrate binding residues, structural features of chymotrypsin, intermediates, and products). Determine Km and Vmax from initial velocity vs. [S] plots or Lineweaver ­Burk (double ­reciprocal) plots. Know the equations that relate the kinetic parameters and be able to calculate kcat, Km, [Et], Vmax, etc. Explain or illustrate effects of pH on enzyme activity (including kcat, KM) with respect to protonation states of important residues in enzymes. (refer to p. 204, 207 of Lehninger and our treatment of chymotrypsin in the lecture notes). Know the maximum value kcat/Km can have for an enzyme in aqueous solution. Know effects on KM and Vmax for reversible inhibitors, including alpha factors. Be able to recognize types of inhibition from effects on velocity vs....
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This test prep was uploaded on 02/25/2014 for the course BIOC 462A taught by Professor Ziegler,baldwin during the Spring '08 term at University of Arizona- Tucson.

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