Ligand concentration plots to identify increases or

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Unformatted text preview: to predict the behavior of proteins in ion exchange chromatography given the pI values. Know the basic structural features common to most amyloid fibrils. Hemoglobin ­know the effects of allosteric regulators (H+, O2, BPG, CO2) on O2 affinity. Use fractional saturation vs. ligand concentration plots to identify increases or decreases in affinity. Understand how stabilization (by regulatory molecules or mutations) of T state or R state affects affinity. Recognize and/or draw cooperative vs. non ­cooperative binding curves or enzyme activity curves. Interpret a value of Kd (or P50) with respect to affinity (i.e., lower value of Kd reflects increased affinity). Use a thermodynamic box to calculate an equilibrium constant and to evaluate the effect of one binding event on another. In enzyme reaction mechanisms, identify the role of functional groups in catalysis (e.g., general or specific acid/base, covalent, metal ion, etc...
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This test prep was uploaded on 02/25/2014 for the course BIOC 462A taught by Professor Ziegler,baldwin during the Spring '08 term at University of Arizona- Tucson.

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